Inhibition of invasiveness of human lung cancer cell line H1299 by over-expression of cofilin |
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Authors: | Lee Yi-Jang Mazzatti Dawn J Yun Zhong Keng Peter C |
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Institution: | Department of Therapeutic Radiology, Yale University, 333 Cedar Street, New Haven, CT 06510, USA. y1343@e-mail.med.yale.edu |
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Abstract: | The Rho-LIM-kinase (LIMK) signaling pathway, believed to be involved in the regulation of tumor invasion, specifically regulates the activity of cofilin. However, it is unclear whether cofilin plays a pivotal role in tumor invasiveness. In this paper we show using a tet-on gene expression system that over-expression of cofilin inhibits the invasiveness of human lung cancer H1299 cells. Over-expressed cofilin disrupts the actin cytoskeleton at the leading edge of the cell and up-regulates p27(kip1), which is known to be involved in regulating cell motility. Removal of cofilin over-expression normalizes the p27(kip1) level and concomitantly restores the invasiveness of the cultured cells. These findings suggest that excessive cofilin production might prevent cancer cell invasion. |
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Keywords: | Cofilin Cancer cell invasion Actin cytoskeleton p27kip1 |
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