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Cloning of the natural gene for the sweet-tasting plant protein thaumatin |
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| Authors: | Adrianus M. Ledeboer C.Theo Verrips Ben M.M. Dekker |
| Affiliation: | a Unilever Research Laboratorium, Olivier van Noortlaan 120, 3133 AT, Vlaardingen, The Netherlands Tel. 010 — 606933 b Department of Medical Biochemistry, Sylvius Laboratories, University of Leiden, Wassenaarseweg 72, Leiden, The Netherlands Tel. 071 — 148333 |
| Abstract: | Five different clones, homologous to the structural gene for the sweet-tasting plant protein thaumatin, have been isolated from leaf DNA of Thaumatococcus daniellii Benth. Restriction maps, hybridization studies, S1-nuclease mapping and R-loop formation revealed that the thaumatin genes isolated belong to one multigene family, and have two very small introns situated at different positions in the various structural genes. A similar situation prevails in a number of seed storage genes. This suggests a similarity between the sweet-tasting protein thaumatin and seed storage proteins. |
| Keywords: | Recombinant DNA Thaumatococcus daniellii plasmids phage λ vector seed storage protein restriction maps introns |
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