Cytochrome P450 1A-dependent enzyme activities in the liver of dab (Limanda limanda): kinetics, seasonal changes and detection limits |
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Authors: | U Lange A Goksyr D Siebers L Karbe |
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Institution: | a Forschungsinstitut Senckenberg, Notkestraße 31, 22607 Hamburg, Germany;b Department of Molecular Biology, University of Bergen, HIB, Thormøhlensgt 55, N-5020 Bergen, Norway;c Alfred-Wegener Institut, Biologische Anstalt Helgoland, Notkestraße 31, 22607 Hamburg, Germany;d Institut für Hydrobiologie und Fischereiwissenschaft, Zeiseweg 9, 22765 Hamburg, Germany |
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Abstract: | Concentrations of total cytochrome P450 and cytochrome P450 1A (CYP 1A) and activities of ethoxycoumarin O-deethylase (ECOD), ethoxyresorufin O-deethylase (EROD) and pentoxyresorufin O-depentylase (PROD) were measured in the liver of prespawning, spawning and postspawning dab (Limanda limanda) from the German Bight. Between all P450-dependent parameters measured significant correlations were found. Generally, during prespawning and spawning season higher values were measured in the liver of males compared to females, but the ratio between sexes changed during spawning time, when concentrations and activities in the liver of males decreased and increased in the liver of females. The activity and the signal-to-noise ratio decrease in the order EROD, ECOD and PROD. This decrease is accompanied by an increase in Km. The findings indicate that the different activities can be attributed to the strongly overlapping substrate specificity and the different enzyme affinities of one enzyme, CYP 1A, towards the three substrates. A biphasic kinetic of ECOD indicates that in addition to CYP 1A a second isozyme catalyses the O-deethylation of ethoxycoumarin in the liver of dab. Interestingly, the ratio between EROD activity and CYP 1A concentration varied seasonally but did not differ significantly between sexes. |
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Keywords: | Cytochrome P450 1A Detection limit ECOD Enzyme kinetic EROD Limanda limanda PROD Sex Stage of maturity |
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