Characterization of Lipopolysaccharides from Ralstonia solanacearum |
| |
Authors: | Varbanets L. D. Vasil'ev V. N. Brovarskaya O. S. |
| |
Affiliation: | (1) Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, Kiev, 252143, Ukraine |
| |
Abstract: | Lipopolysaccharides (LPSs) from four strains of Ralstonia solanacearum belonging to biovar I (ICMP 6524, 8115, 5712, and 8169) were isolated and investigated. The structural components of the LPS molecule, such as lipid A, the core oligosaccharide, and O-specific polysaccharide (O-PS), were obtained after mild acid hydrolysis of the LPS preparations. In lipid A from all the LPS samples studied, 3-hydroxytetradecanoic, 2-hydroxyhexadecanoic, tetradecanoic, and hexadecanoic fatty acids prevailed. The dominant monosaccharides of the core oligosaccharides of all of the strains studied were rhamnose, glucose, glucosamine, 2-keto-3-deoxyoctulosonic acid, and heptose. However, individual strains varied in the content of galactose, ribose, xylose, and arabinose. Three types of the O-PS structure were established, which differed in their configuration ( or ), as well as in the type of the bond between glucosamine and rhamnose residues (1 2 or 1 3). |
| |
Keywords: | Ralstonia solanacearum lipopolysaccharide |
本文献已被 SpringerLink 等数据库收录! |
|