Characterization of a pollen-expressed gene encoding a putative pectin esterase ofPetunia inflata |
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Authors: | Jing-Hong Mu Joseph P. Stains Teh-hui Kao |
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Affiliation: | (1) Department of Biochemistry and Molecular Biology, The Pennsylvania State University, 16802 University Park, PA, USA;(2) Present address: Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, 10021 New York, NY, USA |
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Abstract: | From a pollen tube cDNA library ofPetunia inflata, we isolated cDNA clones encoding a protein, PPE1, which exhibits sequence similarity with plant, bacterial, and fungal pectin esterases. Genomic clones containing thePPE1 gene were isolated using cDNA for PPE1 as a probe, and comparison of the cDNA and genomic sequences revealed the presence of a single intron in thePPE1 gene. During pollen development,PPE1 mRNA was first detected in anthers containing uninucleate microspores; it reached the highest level in mature pollen and persisted at a high level inin vitro germinated pollen tubes. The observed expression pattern of thePPE1 gene suggests that its product may play a role in pollen germination and/or tube growth. |
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Keywords: | pectin esterase Petunia inflata pollen pollen tubes |
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