Induction of acute phase proteins by dexamethasone in rat hepatocyte primary cultures |
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Authors: | V Gross T Andus T A Tran-Thi J Bauer K Decker P C Heinrich |
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Institution: | Biochemisches Institut, Universität Freiburg, D-7800 Freiburg im Breisgau, Germany |
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Abstract: | The effect of dexamethasone on the synthesis of acute phase proteins has been studied in primary cultures of rat hepatocytes. In the absence of dexamethasone no detectable amounts of alpha 2-macroglobulin were synthesized by hepatocytes cultured for 1 day. alpha 2-Macroglobulin synthesis was induced by dexamethasone concentrations of 10(-8) M or higher with a maximum at a concentration of 10(-7) M. alpha 1-Acid glycoprotein was synthesized in the absence of dexamethasone; however, its synthesis was also greatly stimulated by dexamethasone concentrations of 10(-8)-10(-6) M. Synthesis of alpha 1-proteinase inhibitor was stimulated only 1.4-fold at a dexamethasone concentration of 10(-7) M. The kinetics of induction of alpha 2-macroglobulin and alpha 1-acid glycoprotein were studied at a dexamethasone concentration of 10(-7) M. After an initial lag phase of 3 h the synthesis of both proteins showed a steady increase during 2 days. Synthesis of albumin remained unchanged under these experimental conditions. Unlike alpha 2-macroglobulin and alpha 1-acid glycoprotein tyrosine aminotransferase activity increased already during the first 3 h of induction by dexamethasone with a maximum at 12 h followed by a slight decrease. |
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Keywords: | To whom offprint requests should be sent Address: Biochemisches Institut Universität Freiburg Hermann-Herder-Straβe 7 D-7800 Freiburg im Breisgau FRG |
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