Specific chemical modification of cytidine in T4 DNA by a spin label |
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Authors: | S G Kamzolova |
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Abstract: | A procedure for selective modification of DNA from T4 phage non-glucosylated mutant by the spin label--N(2,2',5,5') tetramethyl-3-carboxypyrrolidine-1-oxyl)-imidazole was developed. The spin label was shown to interact with hydroxyl groups of 5-hydroxymethyl-2 deoxycytidines. The modification does not affect the secondary structure of DNA, its conformation or template properties in a cell-free system of RNA synthesis. |
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