The N-glycosylation sites of soybean seed coat peroxidase

Tryptic digestion of apo-soybean peroxidase (apo-SBP), withand without acetamidation, chromatographic separation of thetryptic fragments and MALDI-TOF analysis of the major components,both before and after digestion with glycopeptidase A, demonstratedthe presence of six carbohydrate groups on five peptides. Fiveof the glycopeptides can be mapped with confidence to the peptidescontaining Asn16, Asn90, Asn104, Asn169, and Asn174. The sixthN-glycosylation site is not known and does not appear to beAsn145. It may be present on the N-terminus of SBP, which hasnot been sequenced. soybean seed coat peroxidase glycopeptides glycosylation sites MALDI-TOF MS