A Method for Isolation of Total RNA from Fruit Tissues of Banana |
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Authors: | Jun-Jun Liu Chong-Jin Goh Chiang-Shiong Loh Pei Liu Eng-Chong Pua |
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Affiliation: | (1) Present address: School of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore, 119260, Republic of Singapore |
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Abstract: | We describe a rapid and efficient method for isolation of total RNA from banana fruit tissues. The RNA was extracted with a high ionic strength buffer at room temperature. The proteins, genomic DNA and secondary metabolites in the extract were then removed by precipitation with pre-cooled potassium acetate and repeated phenol/chloroform/isoamyl alcohol extractions. The RNA was recovered by ethanol precipitation. It was relatively free of ribonucleases and was suitable for RT-PCR and northern blot analysis. The procedure can be completed in less than 4 hours. |
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Keywords: | banana fruit tissue Musa sp. RNA isolation |
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