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Detection of Candida sp. mannan antigen by indirect ELISA-inhibition
Authors:J Garcia-de-Lomas  C Morales  M A Grau  A Mir
Institution:(1) Department of Microbiology, Medical School and University Hospital, Valencia, Spain
Abstract:Summary We have obtained mannans from four Candida species: C. albicans A, C. albicans B and C. tropicalis; antimannan sera against C. albicans A, C. albicans B and C. tropicalis were obtained by immunizing rabbits sub-cutaneously with the respective yeast extract. The efficacy of these sera in reacting with mannans obtained from three Candida sp. has been proven by indirect ELISA-inhibition.Any of three immune sera can be used to detect mannan antigen from the three Candida sp. tested. This confirms the existence of crossed reactivity and the possibility of detecting mannan antigen in serum from patients infected by different Candida sp., although we had only one immune serum and one Candida mannan.
Keywords:antigenemia  Candida  mannans  indirect ELISA-inhibition
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