向日葵花盘水提物对硫色镰刀菌及其细胞壁降解酶活性抑制特性

通过抑菌及细胞壁降解酶活性试验,研究向日葵花盘(sunflower disc,SFD)水提物对引起马铃薯干腐病主要病原菌——硫色镰刀菌(Fusarium sulphureum)生长及其侵染不同马铃薯品种时分泌的多聚半乳糖醛酸酶(polygalacturonase,PG)、果胶甲基半乳糖醛酸酶(polymethyl-ga...

Inhibitory characteristics of water extract of sunflower disc on Fusarium sulphureum and its cell wall degrading enzyme activity

By methods of bacteriostatic test and cell-wall degrading enzyme activity test,this study investigated the biological control effect of water extract from sunflower disc on Fusarium sulphureum and the activities of 4 cell-wall degrading enzymes. Polygalacturonase(PG),polymethyl-galacturonase(PMG),carboxymethyl cellulose(Cx)and β-glucosidase were 4 cell-wall degrading enzymes secreted by Fusarium sulphureum invading the different potato varieties. Results show that the extract is of inhibition effects on Fusarium sulphureum and activities of 4 cell-wall degrading enzymes secreted by Fusarium sulphureum invading potato tubers from 3 potato varieties of Longshu No. 7,Longshu No. 10 and Xindaping,and the best inhibition occurrs at the concentration of 320 mg/mL. With the increasing of concentration in the range of 0 to 640 mg/mL,the inhibition effect on Fusarium sulphureum increased. Treated by the extract of the best concentration,the activities of polygalacturonase(PG)and β-glucosidase of Longshu No. 7 can be reduced to the uninfected level,but the reduction of β-glucosidase activity did not achieve a very significant effect. The activities of polymethyl-galacturonase(PMG)and carboxymethyl cellulose(Cx)of Longshu No. 7 and the activities of 4 cellwall degrading enzymes of Longshu No. 10 and Xindaping could not be reduced to uninfected levels.