Detection and characterization of naturally occuring plasmids in Bacillus subtilis |
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| Authors: | Elke Pratje and Georg Michaelis |
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| Affiliation: | (1) Physiologisch-Chemisches Institut der Universität Würzburg, Koellikerstraße 2, D-8700 Würzburg, Federal Republic of Germany;(2) Present address: Fakultät für Biologie, Morgenbreede 45, Universität Bielefeld, Postfach 8640, D-4800 Bielefeld, Federal Republic of Germany |
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| Abstract: | Summary A rapid procedure has been employed to isolate a large number of mitochondrial mutants resistant to antimycin A or funiculosin. A total of 15 antimycin A resistance mutations has been mapped by allelism tests. The mutations belong to two new mitochondrial loci, designated AI and AII. All funiculosin resistance mutations studied up to now map at locus AII. Thus mitochondrial funiculosin resistance might allow the specific selection of mutations in AII. Recombination between the two antimycin A resistance loci AI and AII occurs at frequencies from 8 to 21%. Apparently the two loci are not linked to PAR1, RIB1, RIB3, OLI1, and OLI2. Mutants of the two loci AI and AII have been characterized by measurements of oxygen consumption. Analysis of cytochrome spectra indicates that the mutations affect the cytochrome bc1 complex of the mitochondrial respiratory chain. |
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