Improvement of chloroperoxidase stability by covalent immobilization on chitosan membranes |
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| Authors: | Li-Hua Zhang Chao-Hong Bai Ying-Song Wang Yu-Cheng Jiang Man-Cheng Hu Shu-Ni Li and Quan-Guo Zhai |
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| Institution: | (1) Key Laboratory of Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi’an, Shaanxi, People’s Republic of China;(2) School of Chemistry and Materials Science, Shaanxi Normal University, Chang’an South Road, 199, Xi’an, Shaanxi, 710062, People’s Republic of China |
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| Abstract: | Chloroperoxidase (CPO) from Caldariomyces fumago was optimally covalently immobilized on chitosan membranes pretreated with 0.8 M glutaraldehyde at pH 3.5 to give 3.18 mg
CPO g−1 support. Using monochlorodimedone (MCD) as assay substrate, the immobilized-CPO retained 40% activity at 50°C after 40 min
whereas free CPO retained only 0.02%. The residual activity for immobilized-CPO was 99 and 58% compared with 68 and 43% for
free CPO in the presence of 1.5 M urea and 300 μM H2O2, respectively, after 20 h. |
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| Keywords: | Catalytic activity Chitosan membranes Chloroperoxidase Immobilization Stability |
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