Plantlet formation from embryonic tissue of chestnut grown in vitro |
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Authors: | ANA M. VIEITEZ E. VIEITEZ |
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Affiliation: | Plant Physiology, Instituto de Investigaciones Agrobiológicas de Galicia. C.S.I.C., Apartado 122. Santiago de Compostela, Spain;Department of Plant Physiology, Faculty of Biology, Santiago de Compostela, Spain |
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Abstract: | Development of axillary shoots was induced when isolated embryonic axes of chestnut (Castanea sativa Mill.) were cultured on a defined medium containing 6-benzyl-aminopurine (BAP). The optimal concentrations of BAP were determined for development of axillary shoots from both embryonic axes and subcultured shoots. After shoot multiplication a great number of shoots have been maintained sequentially without significant change in the proliferative rate for one year. Limited rooting has been obtained with excised shoots. Indole-3-butyric acid (IBA) at 2 mg/l was used to induce root primordia. After 8 days of treatment the plantlets were transferred to an auxin-free medium for root development. |
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Keywords: | Castanea sativa chestnut plantlet formation shoot culture in vitro |
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