Mutations affecting translational fidelity in the eucaryote Podospora anserina: Characterization of two ribosomal restrictive mutations |
| |
Authors: | Marguerite Picard-Bennoun |
| |
Institution: | (1) Laboratoire de Génétique de l'Université Paris-Sud, Centre d'Orsay (Laboratoire associé au C.N.R.S. n° 86), Bâtiment 400, F-91405 Orsay, France;(2) Laboratoire de Génétique de l'Université Bordeaux II, Bâtiment 400, F-91405 Orsay, France |
| |
Abstract: | Summary Fifty-nine mutations that restrict suppressor efficiency were selected in the fungus Podospra anserina using four different screening methods. Previous genetic analysis has shown that these antisuppressors lie in six loci and that they could be similar to ribosomal restrictive mutations known in Escherichia coli. The present study deals with the response of two of them, AS1-1 and AS6-1, to paromomycin and low temperature both in vivo and in vitro. The data demonstrate that ribosomes of the mutant and double-mutant strains are equally resistant to the ambiguity effect of paromomycin. These data are the first demonstration of mutations that increase translational fidelity in a eucaryotic organism. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|