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Identification of a 148-kDa surface lectin from Giardia lamblia with specificity for α-methyl-d-mannoside
Authors:K Sreenivas  Nirmal K Ganguly  Sujata Ghosh  Rakesh Sehgal  Ramesh C Mahajan
Institution:Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India; Department of Biotechnology and Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh 160 012, India
Abstract:Abstract A lectin specific for α-methyl-d-mannoside was purified from the membrane extract of Giardia lamblia by a combination of gel filtration chromatography on Sephadex G-75 and Superose 6-HR 10/30. The homogeneity of the lectin was established by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The molecular mass of the native protein was 148 kDa. The lectin agglutinated rabbit erythrocytes in the presence of Ca2+ at 37 °C and pH 7.O. The maximum activity of the lectin was obtained after trypsin treatment. The inhibition study clearly suggests that the binding site of the lectin recognizes α-methyl-d-mannoside as the immunodominant sugar.
Keywords:Lectin              Giardia lamblia            Hemagglutinin  Agglutinin  α-Methyl-d-mannoside  Hemagglutination
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