Isolation, purification, and partial characterization of formate dehydrogenase from soybean seed |
| |
| Authors: | Farinelli M P Fry D W Richardson K E |
| |
| Institution: | The Ohio State University, Department of Physiological Chemistry, Columbus, Ohio 43210. |
| |
| Abstract: | Soybean (Glycine soja var Beeson) formate dehydrogenase has been isolated, purified, and partially characterized by affinity chromatography. The enzyme is a dimer having a total molecular weight of 100,000 and a subunit weight of 47,000. It has activity over a broad pH range, is stable for months at 4°C, and has Km values of 0.6 millimolar and 5.7 micromolar for formate and NAD, respectively. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
