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| 应用微卫星标记进行大豆种质多样性和遗传变异性分析 | |
| 引用本文： | 刘峰,东方阳,陈受宜,邹继军,庄炳昌. 应用微卫星标记进行大豆种质多样性和遗传变异性分析[J]. 遗传学报, 2000, 27(7): 628-633 |
| 作者姓名： | 刘峰 东方阳 陈受宜 邹继军 庄炳昌 |
| 作者单位： | 1. 中国科学院遗传研究所，北京 100101 2. 东北农业大学大豆研究所，哈尔滨 150030 3. 吉林省农业科学院大豆研究所，公主岭 136100 |
| 基金项目： | 国家重大基础项目（G1998010208）和国家863计划（863-101-02-02-03）资助 |
| 摘 要： | 微卫星标记又称ＳＳＲ标记是近年来发展的一种新型的分子标记可有效地进行基因型鉴定，系统谱分析并可估算材料间的遗传距离。用５对ＳＳＲ引物对１５份大豆材料进行扩增，共得到２１条多态性条带。每个ＳＳＲ座位的等侠基因数目为３～＾个，基因多样性范围为０．４３７～０．６６８，对这些材料进行了遗传距离分析。家系分析表明微卫星ＤＮＡ经过多世代的九分裂的后产生了突变，在ＲＩＬ Ｆ８代中有的个体在个别ＳＳＲ等基因的大小 |
| 关 键 词： | 遗传距离 基因多样性 突变频率 大豆 微卫星标记 |
| 文章编号： | 0379-172（2000）07-628-6 |
| 修稿时间： | 1999-08-26 |
Soybean Germplasm Diversity and Genetic Variance Detected by Microsatellite Markers |
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| LIU Feng,DONG Fang-Yang,CHEN Shou-Yi,ZOU Ji-Jun,ZHUANG Bing-Chang. Soybean Germplasm Diversity and Genetic Variance Detected by Microsatellite Markers[J]. Journal of Genetics and Genomics, 2000, 27(7): 628-633 | |
| Authors: | LIU Feng DONG Fang-Yang CHEN Shou-Yi ZOU Ji-Jun ZHUANG Bing-Chang |
| Abstract: | Microsatellite or Simple Sequence Repeats (SSR) marker is a new type of molecular marker developed recently, which can be used in genotype identification, pedigree analysis, and estimation of genetic distance. Using 5 pairs of SSR primer, 21 polymorphic fragments were obtrined in 15 soybean germplasm. The number of alleles at each SSR locus is from 3 to 6, and the range of gene diversity is 0.439~ 0.668. In addition, analysis of genetic distance was also performed in this study.Pedigree analysis showed that mutation occurred for individuals in the F8 population of RIL after multigeneration meiosis, which was caused because of the changes in the number of repeat unit to a limited extent. |
| Keywords: | simple sequence repeat genetic distance gene diversity mutation frequency |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |