Resistance to a DNA and a RNA virus in transgenic plants by using a single chimeric transgene construct |
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| Authors: | Ching-Yi Lin Hsin-Mei Ku Wen-Shi Tsai Sylvia K Green Fuh-Jyh Jan |
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| Institution: | (1) Department of Plant Pathology, National Chung Hsing University, Taichung, 402, Taiwan;(2) Department of Agronomy, National Chung Hsing University, Taichung, 402, Taiwan;(3) Asian Vegetable Research and Development Center—The World Vegetable Center, Tainan, 741, Taiwan; |
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| Abstract: | Tomato leaf curl Taiwan virus (ToLCTWV) and Tomato spotted wilt virus (TSWV) are two major tomato viruses that cause serious economic losses. In this study, a partial C2 gene from ToLCTWV and
the middle half of the N gene of TSWV were fused as a chimeric transgene to develop multiple virus resistance in transgenic
plants. This construct was introduced into Nicotiana benthamiana and tomato by Agrobacterium-mediated transformation. Several transgenic lines showed no symptom post agro-inoculation with ToLCTWV and displayed high
resistance to TSWV. The detection of siRNAs indicated that the resistance was via RNA silencing. This study demonstrated that
linkage of gene segments from two viruses with distinct genomic organization, one DNA and the other RNA, can confer multiple
virus resistance in transgenic plants via gene silencing. |
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