Growing tobacco cells respond to rapid medium changes with a transient increase in localized Ca2+ activity |
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Authors: | Victor A. Vitorello Alfred Haug |
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Affiliation: | (1) Department of Botany and Plant Pathology, Michigan State University, 48824 East Lansing, MI;(2) CENA, Universidade de São Paulo, Piracicaba, SP, Brasil;(3) Department of Microbiology, Michigan State University, 48824 East Lansing, MI, USA |
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Abstract: | Summary A suspension of tobacco cells,Nicotiana tabacum L. BY-2, was subjected to a rapid change of medium, resulting in disturbance of growth. A subpopulation of growing cells responded to such a nutritional signal by establishing a transient, localized Ca2+ accumulation, as judged by chlorotetracycline fluorescence. Residing near or at the plasma membrane, this initial Ca2+ signal began to relax after 1 h to a value presumably corresponding to an equilibrium Ca2+ level. This response was susceptible to treatment with brefeldin A, an agent impacting vesicular traffic, as indicated by a further increase in fluorescence. By contrast, undisturbed growing and non-growing cells did not display a Ca2+ response, regardless of the presence of brefeldin A. |
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