| 家兔肝脏LRP蛋白的分离纯化与鉴定 |
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| 引用本文： | 王璜,赵晓军.家兔肝脏LRP蛋白的分离纯化与鉴定[J].四川动物,2009,28(2). |
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| 作者姓名： | 王璜 赵晓军 |
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| 作者单位： | 四川大学纳米生物医学技术与膜生物学研究所,成都,610041 |
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| 摘 要： | 利用家兔LRP蛋白聚集形成蛋白复合物的性质,首先通过两次蔗糖密度梯度离心从家兔肝脏中分离得到LRP粗提液,再利用分子大小差异,使用Sephacryl-1000分子筛层析纯化,SDS-PAGE银染结果显示最终得到了单一电泳条带的家兔LRP蛋白.最后使用LRP单克隆抗体的Western blotting鉴定结果正确.
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| 关 键 词： | 密度梯度离心 |
Purification and Identification of LRP Protein from Rabbit Livers |
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| WANG Huang,ZHAO Xiao-jun.Purification and Identification of LRP Protein from Rabbit Livers[J].Sichuan Journal of Zoology,2009,28(2). |
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| Authors: | WANG Huang ZHAO Xiao-jun |
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| Institution: | The Institute of Nanobiomedicine Technology and Membrane Biology;Sichuan University;Chengdu 610041;China |
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| Abstract: | In the case of LRP protein assembling into a protein complex,two steps of sucrose density gradient centrifugation were used to separate the LRP protein from rabbit livers.Then followed by the Sephacryl-1000 gel filtration,and after the Ag+ dyeing for SDS-PAGE gel,a perfect single band was shown.The Western blotting using the LRP monoclone antibody was shown a correct result. |
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| Keywords: | LRP Western blotting |
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