<Emphasis Type="Italic">Trichoderma harzianum</Emphasis>: a biocontrol agent against <Emphasis Type="Italic">Bipolaris oryzae</Emphasis> |
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Authors: | Gamal M Abdel-Fattah Yasser M Shabana Adel E Ismail Younes Mohamed Rashad |
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Institution: | (1) Department of Botany, Faculty of Science, Mansoura University, El-Mansoura, 35516, Egypt;(2) Department of Plant Pathology, Faculty of Agriculture, Mansoura University, El-Mansoura, Egypt;(3) Department of Mycological Research and Disease Survey, Institute of Plant Pathology, Agricultural Research Center, Giza, Egypt |
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Abstract: | Rice brown spot, caused by Bipolaris oryzae, can be a serious disease causing a considerable yield loss. Trichoderma harzianum is an effective biocontrol agent for a number of plant fungal diseases. Thus, this research was carried out to investigate
the mechanisms of action by which T. harzianum antagonizes Bipolaris oryzae in vitro, and the efficacy of spray application of a spore suspension of T. harzianum for control of rice brown spot disease under field conditions. In vitro, the antagonistic behavior of T. harzianum resulted in the overgrowth of B. oryzae by T. harzianum, while the␣antifungal metabolites of T.␣harzianum completely prevented the linear growth of B. oryzae. Light and scanning electron microscope (SEM) observations showed no evidence that mycoparasitism contributed to the aggressive
nature of the tested isolate of T. harzianum against B. oryzae. Under field conditions, spraying of a spore suspension of T. harzianum at 108 spore ml−1 significantly reduced the disease severity (DS) and disease incidence (DI) on the plant leaves, and also significantly increased
the grain yield, total grain carbohydrate, and protein, and led to a significant increase in the total photosynthetic pigments
(chlorophyll a and b and carotenoids) in rice leaves. |
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Keywords: | Antagonism Antifungal Biological control Brown spot Mycoparasitism Rice |
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