Secretion and localization of invertase and inulinase in recombinant Saccharomyces cerevisiae |
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| Authors: | Soo-Wan Nam Koji Yoda Makari Yamasaki |
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| Institution: | (1) Genetic Engineering Research Institute, KIST, P.O. Box 17, Taejon, Korea;(2) Dept. of Agricultural Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113 Tokyo, Japan |
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| Abstract: | Summary Secretion of invertase and inulinase produced by recombinant Saccharomyces cerevisiae cells were investigated under derepression conditions of GALI promoter. Secreted invertase mainly localized in the periplasmic space, but most of inulinase was found in the extracellular culture medium. This high level of extracellular secretion of inulinase was not dependent on the growth phase in which derepression of GALI promoter occurs. Our results indicate that the inulinase polypeptide itself may have a function for the protein secretion into the culture medium. |
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