The energy dispersive X-ray microanalysis technique in the study of fluids and tissues of the brain and the inner ear |
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Authors: | M. Anniko R. Wroblewski |
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Affiliation: | (1) Department of Otolaryngology, Karolinska sjukhuset, S-104 01 Stockholm, Sweden;(2) King Gustaf V Research Institute, Karolinska Institute, S-104 01 Stockholm, Sweden;(3) The Wenner-Gren Institute, University of Stockholm, S-113 45 Stockholm, Sweden;(4) Department of Physiology II, Karolinska Institute, S-104 01 Stockholm, Sweden |
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Abstract: | Summary The temporal bone from the fetal and adult mouse and brain from fetal and adult rat were cryosectioned in a conventional cryostat at –30° C using 16 m thick sections. The elemental composition was analyzed in the fluid-filled spaces of the membraneous labyrinth and ventricular cavities of the brain. After drying these spaces contained small crystal-like aggregations of material which morphologically differed depending on their elemental composition. This changed during embryonic maturation. The qualitative X-ray analytical microscopy seems to be a reliable method to follow changes in the elemental composition of fluids.Supported by grant no 12X-720 from the Swedish Medical Research Council. Doctor Wroblewski was during the course of this investigation supported by a research fellowship from the Muscular Dystrophy Association, USA |
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