长叶榧(Torreya jackii Chun)遗传多样性分析

利用RAPD技术分析了长叶榧(Torreya jackii Chun)的遗传结构,用18个引物对来自福建省泰宁县的3个不同居群的66个长叶榧样本进行分析.结果显示,长叶榧群体水平的多态位点百分率达87%,居群水平的多态位点百分率分别为83%、68%和80%.群体水平上的Nei基因多样性指数为0.247 9,居群水平上分别为0.218 2、0.168 4和0.188 1.群体水平上的平均杂合度达到0.244 7,居群水平上分别为0.221 5、0.172 9和0.195 0.30.35%的遗传变异存在于居群间,69.65%的遗传变异存在于居群内.长叶榧居群的基因流量为1.689 9,显示长叶榧仍具有通过群体间的基因交流来防止遗传漂变造成的群体分化的能力.

Analysis of genetic diversity for Torreya jackii Chun by RAPD

The genetic diversity of Torreya jackii Chun was researched by RAPD. 18 primers were screened to amplify the genome of 66 samples of T. jackii from 3 populations in Taining County of Fujian Province. These primers detected 199 reproducible fragments among which there were 173 reproducible polymorphism fragments. The results showed that the percentage of polymorphic site at species level was 87%, and they were 83%, 68% and 80% at population levels respectively. Nei's gene diversity index was 0.247 9 at species level, and 0.218 2, 0.168 4 and 0.188 1 at population levels respectively. The heterozygosity was 0.244 7 at species level, and 0.221 5, 0.172 9 and 0.195 0 respectively at population levels. The percentage of genetic variance was 30.35% among T. jackii populations, and 69.65% within population. The gene flow index of the species was 1.689 9, it was suggested that the species still has the ability to recover from its endangered situation through promoting the genetic communication among populations.