簇毛麦染色体组特异性RAPD标记的筛选、定位和应用

以普通小麦中国春、中国春－簇毛麦二体附加系以及不同来源的簇毛麦为材料，用100个10碱基随机引物进行RAPD扩增。引物OPF02能在不同来源的簇毛麦及所有中国春－簇毛麦二体附加系中扩增出一条长约750bp的片段OPF02 750。普通小麦和硬粒小麦不能扩增出该片段。因此，OPF02 750为分布于簇毛麦所有染色体上的一个簇毛麦染色体组特异片段。用引物OPF02对普通小麦－簇毛麦双二倍体、硬粒小麦－簇毛麦双二倍体以及几个普通小麦的簇毛麦二体代换系、二体附加系进行检测，发现NAU302已经丢失了其所附加的簇毛麦3V染色体。

Identification, Mapping, and Application of A Chromosome-specific RAPD Marker from Haynaldia villosa

Random amplified polymorphic DNA (RAPD) analysis was performed on common wheat of Chinese Spring, addition lines of H. villosa chromosome in CS and H.villosum from different accessions with 100 random 10 base primers. A chromosome specific polymorphic DNA segment for H.villosa , OPF02 750 , was obtained from all addition lines of H.villosa chromosome in CS and H. villosum which belong to different accessions. The result amplified by primer OPF02 of all addition lines of H. villosa chromosome in CS indicated that all the seven pairs of H. villosa chromosomes contain OPFO2 750 segment. There was no OPF02 750 in all Triticum aestivum and T. durum tested. Using OPF02, We confirmed that NAU302, an addition line of H. villosa chromosome 3V, had lost its chromosome 3V of H. villosa . Therefore, OPF02 750 is specific to chromosomes of H.villosa ,and could be used as a molecular marker for detection of chromosome of H.villosa in wheat.