Comparative field performance over 3 years and two sites of transgenic wheat lines expressing HMW subunit transgenes |
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| Authors: | Peter R Shewry Stephen Powers J Michael Field Roger J Fido Huw D Jones Gillian M Arnold Jevon West Paul A Lazzeri Pilar Barcelo Francisco Barro Arthur S Tatham Frank Bekes Barbara Butow Helen Darlington |
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| Institution: | (1) Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK;(2) Advanta Seeds UK Ltd, Docking, Norfolk, PE31 8LS, UK;(3) Long Ashton Research Station, Long Ashton, Bristol, BS41 9AF, UK;(4) CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2600, Australia;(5) Present address: Oryzon Genomics, Parc Cientific de Barcelona, Joseph Samitier 1-5, 08028 Barcelona, Spain;(6) Present address: Agrasys SL, Enrique Granados 111, Aytico 2, 08008 Barcelona, Spain;(7) Present address: Instituto de Agricultura Sosterisible, CSIC, Apdo 4084, 14080 Cordoba, Spain;(8) Present address: Biomedical Sciences, Dstl, Porton Down, Salisbury, Wiltshire, SP4 0JQ, UK;(9) Present address: Food Standards Australia New Zealand, PO Box 7186, Canberra, ACT, 2610, Australia |
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| Abstract: | A series of transgenic wheat lines expressing additional high molecular weight (HMW) subunit genes and the corresponding control lines were grown in replicate field trials at two UK sites (Rothamsted Research, approximately 50 km north of London and Long Ashton, near Bristol) over 3 years (1998, 1999, 2000), with successive generations of the transgenic lines (T3, T4, T5) being planted. Four plots from each site were used to determine grain dry weight, grain nitrogen, dough strength (measured as peak resistance by Mixograph analysis) and the expression levels of the endogenous and “added” subunits. Detailed statistical analyses showed that the transgenic and non-transgenic lines did not differ in terms of stability of HMW subunit gene expression or in stability of grain nitrogen, dry weight or dough strength, either between the 3 years or between sites and plots. These results indicate that the transgenic and control lines can be regarded as substantially equivalent in terms of stability of gene expression between generations and environments. |
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