Flow cytometry applications in the food industry |
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Authors: | Jaume Comas-Riu Núria Rius |
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Affiliation: | (1) Serveis Cientificotècnics, Universitat de Barcelona, c/ Baldiri Reixac 10, 08028 Barcelona, Spain;(2) Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmacia, Universitat de Barcelona, Avda. Joan XXIII s/n, 08028 Barcelona, Spain |
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Abstract: | Flow cytometry has become a valuable tool in food microbiology. By analysing large numbers of cells individually using light-scattering and fluorescence measurements, this technique reveals both cellular characteristics and the levels of cellular components. Flow cytometry has been developed to rapidly enumerate microorganisms; to distinguish between viable, metabolically active and dead cells, which is of great importance in food development and food spoilage; and to detect specific pathogenic microorganisms by conjugating antibodies with fluorochromes, which is of great use in the food industry. In addition, high-speed multiparametric data acquisition, analysis and cell sorting, which allow other characteristics of individual cells to be studied, have increased the interest of food microbiologists in this technique. This mini-review gives an overview of the principles of flow cytometry and examples of the application of this technique in the food industry. |
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Keywords: | Flow cytometry Food microbiology |
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