Lifelong reduction in complex IV induces tissue‐specific metabolic effects but does not reduce lifespan or healthspan in mice |
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Authors: | Sathyaseelan S Deepa Gavin Pharaoh Michael Kinter Vivian Diaz Wilson C Fok Kaitlyn Riddle Daniel Pulliam Shauna Hill Kathleen E Fischer Vanessa Soto Constantin Georgescu Jonathan D Wren Carlo Viscomi Arlan Richardson Holly Van Remmen |
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Institution: | 1. Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma;2. Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma;3. Department of Cellular and Structural Biology, Barshop Institute for Longevity and Aging Studies, University of Texas Health Science Center San Antonio, San Antonio, Texas;4. Division of Hematology, Department of Medicine, Washington University in St. Louis, St. Louis, Missouri;5. Biology Department, University of Alabama at Birmingham, Birmingham, Alabama;6. Arthritis & Clinical Immunology Research Program, Division of Genomics and Data Sciences, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma;7. MRC‐Mitochondrial Biology Unit, University of Cambridge, Cambridge, UK;8. Department of Geriatric Medicine, Reynolds Oklahoma Center on Aging, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma;9. Oklahoma City VA Medical Center, Oklahoma City, Oklahoma |
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Abstract: | Loss of SURF1, a Complex IV assembly protein, was reported to increase lifespan in mice despite dramatically lower cytochrome oxidase (COX) activity. Consistent with this, our previous studies found advantageous changes in metabolism (reduced adiposity, increased insulin sensitivity, and mitochondrial biogenesis) in Surf1?/? mice. The lack of deleterious phenotypes in Surf1?/? mice is contrary to the hypothesis that mitochondrial dysfunction contributes to aging. We found only a modest (nonsignificant) extension of lifespan (7% median, 16% maximum) and no change in healthspan indices in Surf1?/? vs. Surf1+/+ mice despite substantial decreases in COX activity (22%–87% across tissues). Dietary restriction (DR) increased median lifespan in both Surf1+/+ and Surf1?/? mice (36% and 19%, respectively). We measured gene expression, metabolites, and targeted expression of key metabolic proteins in adipose tissue, liver, and brain in Surf1+/+ and Surf1?/? mice. Gene expression was differentially regulated in a tissue‐specific manner. Many proteins and metabolites are downregulated in Surf1?/? adipose tissue and reversed by DR, while in brain, most metabolites that changed were elevated in Surf1?/? mice. Finally, mitochondrial unfolded protein response (UPRmt)‐associated proteins were not uniformly altered by age or genotype, suggesting the UPRmt is not a key player in aging or in response to reduced COX activity. While the changes in gene expression and metabolism may represent compensatory responses to mitochondrial stress, the important outcome of this study is that lifespan and healthspan are not compromised in Surf1?/? mice, suggesting that not all mitochondrial deficiencies are a critical determinant of lifespan. |
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Keywords: | cytochrome c oxidase dietary restriction lifespan mitochondria SURF1 mitochondrial unfolded protein response |
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