Ca(2+) channels activated by endothelin-1 in CHO cells expressing endothelin-A or endothelin-B receptors |
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Authors: | Kawanabe Y Okamoto Y Enoki T Hashimoto N Masaki T |
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Affiliation: | Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606-8507, Japan. kawanabe@kuhp.kyoto-u.ac.jp |
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Abstract: | We comparedthe Ca2+ channels activated by endothelin-1 (ET-1) inChinese hamster ovary (CHO) cells stably expressing endothelin type A(ETA) or endothelin type B (ETB) receptorsusing the Ca2+ channel blockers LOE-908 and SK&F-96365. Inboth CHO-ETA and CHO-ETB, ET-1 at 0.1 nMactivated the Ca2+-permeable nonselective cation channel-1(NSCC-1), which was sensitive to LOE-908 and resistant to SK&F-96365.ET-1 at 1 nM activated NSCC-2 in addition to NSCC-1; NSCC-2 wassensitive to both LOE-908 and SK&F-96365. ET-1 at 10 nM activated thesame channels as 1 nM ET-1 in both cell types, but inCHO-ETA, it additionally activated the store-operatedCa2+ channel (SOCC), which was resistant to LOE-908 andsensitive to SK&F-96365. Up to 1 nM ET-1, the level of the formation of inositol phosphates (IPs) was low and similar in both cell types, but,at 10 nM ET-1, it was far greater in CHO-ETA than inCHO-ETB. These results show that, in CHO-ETAand CHO-ETB, ET-1 up to 10 nM activated the sameCa2+ entry channels: 0.1 nM ET-1 activated NSCC-1, andET-1 1 nM activated NSCC-1 and NSCC-2. Notably, inCHO-ETA, 10 nM ET-1 activated SOCCs because of the higherformation of IPs. |
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