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Determination of a Non-methylated Deoxycytidine Residue in the Recognition Site of DNA-methyltransferases |
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| Authors: | E A Kubareva J Walter O V Vorob'eva M V Razumikhin A S Karyagina P C K Lau T Trautner |
| Institution: | (1) Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119899, Russia;(2) Max-Planck-Institut für Moleculare Genetic, Ihnestrasse 73, BerlinO, D-14195, Germany;(3) Institute of Agricultural Biotechnology, Timiryazevskaya ul. 42, Moscow, 127550, Russia;(4) Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec H4P 2R2, Canada |
| Abstract: | A method for determination of a non-methylated deoxycytidine (dC) residue in the recognition site of 5-cytosine DNA-methyltransferases is suggested. The method is based on treatment of methylated DNA by sodium bisulfite and successive reaction of the thus modified DNA with a repair enzyme, uracil-DNA glycosylase. This method was successfully applied to identify NlaX methyltransferase specificity. |
| Keywords: | 5-cytosine DNA-methyltransferase non-methylated and methylated bases bisulfite reaction uracil-DNA glycosylase |
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