The araBAD operon of Salmonella typhimurium LT2. II. Nucleotide sequence of araA and primary structure of its product, L-arabinose isomerase |
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Authors: | H C Lin S P Lei G Wilcox |
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Institution: | Department of Microbiology and Molecular Biology Institute, University of California, Los Angeles, CA 90024 U.S.A. Tel. (213) 825-0655 |
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Abstract: | The nucleotide sequence of gene araA of Salmonella typhimurium LT2 has been determined. The gene encodes an L-arabinose isomerase (EC 5.3.1.4) of 500 amino acid residues with a calculated Mr of 55814. The ATG start codon of araA is 10 bp distal to the TAA termination codon of araB. A presumed ribosome-binding site (RBS) "TAAGGA" 7 bp from the ATG codon overlaps the stop codon of araB. L-Arabinose isomerase was purified and the amino acid composition is in agreement with that predicted from the DNA sequence. The NH2-terminus of the protein is modified as the sequence cannot be analyzed by the automated Edman degradation. Amino acid composition analyses of both NH2-terminal and C-terminal cyanogen bromide (CNBr) cleaved peptides and partial amino acid sequence of the C-terminal peptide are consistent with the deduced amino acid sequence. |
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Keywords: | Recombinant DNA protein purification codon bp base pairs CNBr cyanogen bromide isomerase HPLC high-performance liquid chromatography ORF open reading frame RBS ribosomebinding site SDS sodium dodecyl sulfate [] indicates plasmidcarrier state |
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