The stress-70 protein family in diplopods: induction and characterization |
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Authors: | M Zanger G Alberti M Kuhn H -R Köhler |
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Institution: | (1) Zoological Institute I (Morphology and Ecology), University of Heidelberg, Im Neuenheimer Feld 230, D-69120 Heidelberg, Germany;(2) Department of Molecular Genetics, University of Heidelberg, Im Neuenheimer Feld 230, D-69120 Heidelberg, Germany |
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Abstract: | To induce stress-70 proteins (hsp70), adults of the millipede Julus scandinavius (Diplopoda) were exposed to leaf litter contaminated with different concentrations of Cd2+ (10, 30, 50 and 60 mg·kg-1 as CdCl2). The expression of hsp70 was investigated by semiquantitative and qualitative biochemical methods. After SDS-gel electrophoresis and Western blotting a subsequent digital image analysis showed that increasing dietary concentrations of Cd2+ resulted in elevated levels of hsp70, which in turn indicated proteotoxic condition. Qualitative results were obtained by two-dimensional gel electrophoresis. A stress-70 protein family, similar to that of other arthropods, was detected in Julus scandinavius: at least five different proteins with an approximate molecular weight of 68, 69, 70, 77, and 78 kDa could be distinguished after heat shock as well as after Cd2+ exposure.Abbreviations
IEF
isoelectric focusing
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hsp
heat shock protein(s)
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grp
glucose regulated protein(s)
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PAGE
polyacrylamide gel electrophoresis
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SDS
sodium dodecyl sulphate |
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Keywords: | Cadmium Stress-70 hsp70 Two-dimensional gel electrophoresis Diplopod Julus scandinavius |
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