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A Comparative Study of Nitrilases Identified by Genome Mining |
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| Authors: | Ond?ej Kaplan Alicja B Veselá Alena Pet?í?ková Fabrizia Pasquarelli Martina Pi?manová Anna Rinágelová Tek Chand Bhalla Miroslav Pátek Ludmila Martínková |
| Institution: | 1. Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 14220, Prague, Czech Republic 5. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovského nám 2, 162 06, Prague, Czech Republic 2. Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 12840, Prague, Czech Republic 3. Department of Chemical Engineering, University of L’Aquila, via Campo di Pile, 67100, L’Aquila, Italy 4. Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, 171005, Himachal Pradesh, India |
| Abstract: | Escherichia coli strains expressing different nitrilases transformed nitriles or KCN. Six nitrilases (from Aspergillus niger (2), A. oryzae, Neurospora crassa, Arthroderma benhamiae, and Nectria haematococca) were arylacetonitrilases, two enzymes (from A. niger and Penicillium chrysogenum) were cyanide hydratases and the others (from P. chrysogenum, P. marneffei, Gibberella moniliformis, Meyerozyma guilliermondi, Rhodococcus rhodochrous, and R. ruber) preferred (hetero)aromatic nitriles as substrates. Promising nitrilases for the transformation of industrially important substrates were found: the nitrilase from R. ruber for 3-cyanopyridine, 4-cyanopyridine and bromoxynil, the nitrilases from N. crassa and A. niger for (R,S)-mandelonitrile, and the cyanide hydratase from A. niger for KCN and 2-cyanopyridine. |
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