Neurocytoprotective effects of the bioactive constituents of Pueraria thomsonii in 6-hydroxydopamine (6-OHDA)-treated nerve growth factor (NGF)-differentiated PC12 cells |
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| Authors: | Chien-Min Lin Rong-Dih Lin Shui-Tein Chen Yi-Pei Lin Wen-Ta Chiu Jia-Wei Lin Feng-Lin Hsu Mei-Hsien Lee |
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| Affiliation: | 1. Graduate Institute of Clinical Medicine, Taipei Medical University, Taipei 110, Taiwan;2. Department of Neurosurgery, Taipei Medical University – Wan Fang Hospital, Taipei 116, Taiwan;3. Department of Neurosurgery, Taipei Medical University – Shuang Ho Hospital, Taipei County 235, Taiwan;4. Department of Internal Medicine, Ho-Ping Branch, Taipei City Hospital, Taipei 100, Taiwan;5. Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 115, Taiwan;6. Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei 100, Taiwan;7. Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan;8. Center for Reproductive Medicine and Sciences, Taipei Medical University Hospital, Taipei 110, Taiwan |
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| Abstract: | Chronic neurodegenerative disorders are having an increasing impact on public health as human longevity increases. Parkinson’s disease (PD) is a degenerative disorder of the central nervous system and is characterized by motor system disorders resulting in loss of dopamine-producing brain cells. Pueraria thomsonii Benth. (Fabaceae) is an herbal medicine that has traditionally been used as an antipyretic agent. In the present study, the active constituents, daidzein and genistein, were isolated from P. thomsonii. Both compounds exhibited neurocytoprotective effects against 6-hydroxydopamine (6-OHDA)-induced cytotoxicity in nerve growth factor (NGF)-differentiated PC12 cells. Neither daidzein nor genistein affected 6-OHDA-induced cellular reactive oxygen species (ROS) generation according to flow cytometric analysis. Rather, they inhibited caspase-8 and partially inhibited caspase-3 activation, providing a protective mechanism against 6-OHDA-induced cytotoxicity in NGF-differentiated PC12 cells. The present results imply that daidzein and genistein may be useful in the development of future strategies for the treatment of PD. |
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