Glomus fasciculatum alleviates transplantation shock of micropropagated Sesbania sesban |
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Authors: | S. Subhan P. Sharmila P. Pardha Saradhi |
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Affiliation: | (1) Centre for Biosciences, Jamia Millia Islamia, New Delhi 110025, India Fax no.: +91-11-682132 E-mail: pardh.bi@jmi.ernet.in, IN |
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Abstract: | Investigations were carried out using the vesicular arbuscular mycorrhizal fungus, Glomus fasciculatum, to improve the success in transplanting micropropagated plantlets of Sesbania sesban. Plantlets were developed from somatic embryos and/or adventitious buds (induced from various explants on Gamborg's medium supplemented with 6-benzylaminopurine), in the presence of 10–7 m α-naphthaleneacetic acid and 5×10–6 m gibberellic acid. Subsequent to nodulating the roots with Rhizobium, plantlets were transplanted into sterile garden soil and inoculated with or without G. fasciculatum. Only 30% of plantlets transferred to soil without G. fasciculatum survived. In contrast, all the plantlets inoculated with G. fasciculatum survived. Histochemical study revealed the presence of intracellular hyphae with well-developed arbuscules and intercellular hyphae with vesicles, suggesting that G. fasciculatum formed a good mycorrhizal association with S. sesban roots. These observations showed that mycorrhizal association helped to increase the potential of micropropagated plantlets to successfully withstand transplantation shock. Received: 6 January 1997 / Revision received: 28 August 1997 / Accepted: 5 September 1997 |
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Keywords: | Glomus fasciculatum Sesbania sesban Micropropagation Vesicular arbuscular mycorrhiza (VAM) Transplantation shock |
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