Use of methicillin and ampicillin mixture as a selective pressure in the cultivation of recombinant E. coli |
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Authors: | Dong-Cheol Lee Hak-Sung Kim |
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Institution: | (1) Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Kusung-Dong, 305-701 Yusung-Gu Taejon, Korea |
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Abstract: | Summary Ampicillin was rapidly degraded by an extracellular -lactamase, and was therefore ineffective to isolate plasmid-harboring cells in the cultivation of recombinant E. coli for the production of thermostable d-hydantoinase. An effective way of preventing the degradation of ampicillin, methicillin was employed as a -lactamase inhibitor. A mixture of methicillin and ampicillin was observed to effectively function as a selective pressure, and consequently plasmid stability and enzyme productivity of recombinant E. coli were highly maintained. |
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