Histochemical analysis of sialic acids in the epididymis of the rat |
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Authors: | T Ueda Osamu Fujimori Azuma Tsukise Kazuyori Yamada |
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Institution: | (1) 2nd Department of Anatomy, Nagoya City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-0001, Japan Tel.: +81-52-853-8126; fax: +81-52-852-8887; e-mail: tueda@med.nagoya-cu.ac.jp, JP;(2) Department of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, Japan , JP |
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Abstract: | A variety of sialic acids contained in the rat epididymis were histochemically examined by means of lectin and pre-lectin
methods by light microscopy. Epididymides from adult male Sprague-Dawley rats were fixed in Bouin’s fluid and routinely embedded
in paraffin wax. Hydrated sections were subjected either to the lectin methods using biotinylated Limax flavus, Sambucus nigra, Sambucus sieboldiana or Maackia amurensis lectins or to the selective periodate oxidation–phenylhydrazine–thiocarbohydrazide–silver protein–physical development technique
with or without saponification. The present results revealed that principal cells in the initial segment and caput contain
sialic acid linked to α2,6-galactose/N-acetylgalactosamine, whereas those in the corpus and cauda include the sialic acidα2,3-galactose sequence. Narrow and clear
cells involve all the types of sialic acids examined. Basal and halo cells mainly contain sialic acidα2,3-galactose. 8- And/or
9-O-acetylated sialic acids were predominantly distributed in principal cells of the initial segment and proximal caput. These
findings are taken to indicate that various sialic acids in the epididymis could participate in different physiological functions
characteristic of the regions in this organ.
Accepted: 10 November 1997 |
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