Expression of a hybrid immunoglobulin-T cell receptor protein in transgenic mice |
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Authors: | M L Becker R Near M Mudgett-Hunter M N Margolies R T Kubo J Kaye S M Hedrick |
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Affiliation: | Department of Biology and Cancer Center, University of California at San Diego, La Jolla 92093. |
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Abstract: | We have constructed a hybrid immunoglobulin (VDJH)-T cell receptor (C alpha) gene using the VDJH exon from a digoxin-specific antibody. This gene was used to make a line of transgenic mice. The hybrid VDJH-C alpha protein is expressed on a subset of T cells in these mice, and we have shown that it forms part of a functional TCR complex by the criteria of coprecipitation and comodulation of CD3 and TCR beta chain components and T cell activation with anti-idiotypic antibodies or digoxin. Furthermore, in cells expressing the hybrid protein, there is allelic exclusion of endogenous TCR alpha genes. We discuss the implications for the comparative structure of T cell receptors and immunoglobulins. |
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