Cloning and different expression of ATP synthase genes between propargite resistant and susceptible strains of Tetranychus cinnabarinus (Acarina: Tetranychidae) |
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| Authors: | Yanjie Luo Jing Ni Kuanyu Zheng Zhenguo Yang Daoyan Xie Aisi Da Jianping Chai Xiujun Jiang Shaoxiang Li |
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| Institution: | 1. Sericulture and Apiculture Institute, Yunnan Academy of Agricultural Sciences, Mengzi 661101, China;2. Yunnan Province Key Laboratory of Agricultural Biotechnology, Biotechnology and Germplasm Resource Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China;3. Institute of Crop, Yunnan Academy of Agricultural Sciences, Kunming 650205,China |
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| Abstract: | The carmine spider mite, Tetranychus cinnabarinus (Boisduval), is a serious phytophagous mite damaging important crops and can rapidly develop resistance to acaricides. Mitochondrial ATP synthase (F1F0 ATP synthase) is an important target site of acaricides. The role of ATP synthase in acaricide resistance remains unclear at the molecular level. In this study, twelve full-length cDNAs of ATP synthase genes were cloned and characterized from T. cinnabarinus and their expression levels were determined for both progargite-resistant and susceptible strains. The effect of propargite exposure on gene expression was also evaluated. Analyses of gene expression revealed that TcATPsynU-2, TcATPsynF0-2 and TcATPsynF0-4 were significantly down-regulated in the progargite-resistant strain. TcATPsynF0-2 and TcATPsynF0-4 had a strong response to progargite exposure. The results suggest that lower levels of TcATPsynU-2, TcATPsynF0-2 and TcATPsynF0-4 expression might be related to propargite-resistance observed in the resistant T. cinnabarinus. This is the first attempt to identify specific ATPase genes involved in propargite resistance in T. cinnabarinus. |
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| Keywords: | Mitochondria ATP synthase Propargite Acaricide resistance Gene expression |
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