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Long-term survival of haptophyte and prasinophyte resting stages in marine sediment |
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| Authors: | Marianne Ellegaard Øjvind Moestrup Thorbjørn Joest Andersen Nina Lundholm |
| Affiliation: | 1. Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmarkme@plen.ku.dk;3. Department of Biology, University of Copenhagen, Universitetsparken 4, DK-2100 Copenhagen ?, Denmark;4. Department of Geosciences and Natural Resource Management, University of Copenhagen, ?ster Voldgade 10, 1350 Copenhagen K, Denmark;5. Natural History Museum of Denmark, University of Copenhagen, S?lvgade 83S, DK-1307 Copenhagen K, Denmark |
| Abstract: | Resting stages of marine phytoplankton have been shown to have potential for long-term survival and to remain viable in marine sediments for up to about a century. This study documents for the first time long-term survival in haptophytes and prasinophytes, by germination of resting stages of Isochrysis galbana and Mantoniella squamata from up to 40-year-old sediment layers. Germination was induced by setting up sediment slurries in L1 medium at 15°C. Cyst formation was induced in culture strains acquired from the germinations by keeping mixtures of strains in the light or dark at salinities of 20 or 30. The identity of the two species was confirmed by light and electron microscopy as well as LSU and SSU rDNA-based phylogenetic analyses. |
| Keywords: | cyst Isochrysis life-cycle Mantoniella phytoplankton resting stage |