Serum-free medium conditions for steroidogenesis of bovine follicular thecal cells cultured on collagen gel matrix |
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Authors: | Hodaka Ikeda |
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Institution: | (1) Department of Biochemistry, Kitasato University School of Medicine, 1-15-1 Kitasato, 228 Sagamihara-shi, Kanagawa, Japan |
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Abstract: | Summary Thecal cells isolated from bovine ovarian follicles were cultured with a serum-free basal medium or a serum-free complete
medium in the presence or absence of collagen gel matrix, and their cellular proliferation and steroidogenesis were compared
with those of cells cultured with a serum-containing medium. The cells cultured with the serum-free basal medium produced
larger amounts of progesterone, androstenedione, and estradiol than the cells cultured with the serum-containing medium, but
no appreciable cell proliferation was observed in the serum-free medium. Response of thecal cells to 8 bromo-cAMP, a steroidogenic
agent, varied according to the type of steroid production examined and the type of culture medium used. In a cultivation period
of 4 d, progesterone production was stimulated about five-fold by 8 bromo-cAMP in the serum-free complete medium on collagen
gel matrix and in the serum-free basal medium without collagen matrix, whereas androstenedione production was stimulated about
three- to fourfold in the serum-free complete medium on collagen gel matrix and in the serum-free basal medium with or without
collagen matrix. Estradiol production, however, was significantly suppressed by 8 bromo-cAMP in the serum-free complete medium
on collagen gel matrix and also in the serum-containing medium. Thus, among the conditions examined, the most suitable primary
culture media for steroidogenesis of thecal cells were the serum-free media, especially serum-free complete medium on collagen
gel matrix. |
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Keywords: | thecal cells serum-free medium steroidogenesis culture on collagen gel matrix cyclic-AMP |
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