Drug-resistant mutants of chinese hamster ovary cells possess an altered cell surface carbohydrate component

Surface label experiments using the galactose oxidase-³ H] -borohydride technique reveal that cells from drug-resistant Chinese hamster ovary clones possess a surface carbohydrate component of apparent molecular weight 165,000 which is absent from wild-type cells. The component may also be demonstrated by ¹⁴C] glucosamine incorporation but not by ³ H] leucine incorporation or by the lactoperoxidase surface labeling reaction.