Strong genetic differentiation of the East-Himalayan <Emphasis Type="Italic">Megacodon stylophorus</Emphasis> (Gentianaceae) detected by Inter-Simple Sequence Repeats (ISSR) |
| |
Authors: | X?-J?Ge L?-B?Zhang Email author" target="_blank">Y?-M?YuanEmail author G?Hao T?-Y?Chiang |
| |
Institution: | (1) South China Botanical Garden, Chinese Academy of Sciences, 510650 Guangzhou, P.R. China;(2) Institut de Botanique, Université de Neuchâtel, Emile-Argand 11, CH-2007, Neuchâtel, Switzerland;(3) Department of Biology, Cheng-Kung University, 701 Tainan, Taiwan |
| |
Abstract: | Megacodon stylophorus (Clarke) Smith is a perennial alpine herb endemic to the species-rich eastern Himalayan region. Its populations are locally scattered as isolated patches throughout this region. Genetic variation within and among six populations of this species was assessed using ISSR fingerprinting with 13 primers. High levels of genetic diversity exist within species (P = 69.83%, HT = 0.1949 and Hsp = 0.3047), while the within-population diversity is low (P = 11.21%, HE = 0.0532 and Hpop = 0.0792). Extraordinarily high levels of genetic differentiation were detected among populations based on various statistics, including Neis genetic diversity analysis (72.7%), Shannons diversity index (74.01%) and AMOVA (80.70%). That is, populations shared low levels of genetic identity (I = 0.8203 ± 0.0430). This genetic structure was probably due to severe genetic drift of the small-sized patchy populations resulting from postglacial habitat fragmentations. The observed genetic structure of the populations implies that as many populations as possible should be considered for any in situ and ex situ conservation practice on this species. |
| |
Keywords: | East Himalaya Genetic diversity Genetic drift Hengduan Mountains ISSR Megacodon stylophorus |
本文献已被 SpringerLink 等数据库收录! |
|