| 转小麦冷胁迫蛋白截短基因烟草及其抗病性分析 |
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| 作者姓名： | Li N DU XZ Pan XM Wang JS Song CF |
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| 作者单位： | 南京农业大学植物保护学院,农作物生物灾害综合治理教育部重点实验室,南京,210095 |
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| 基金项目： | 国家自然科学基金项目,江苏高校优势学科建设工程项目资助 |
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| 摘 要： | TA3-13是克隆于小麦冷胁迫蛋白基因的截短片段。原核表达的TA3-13蛋白能够诱导烟草产生显著的抗烟草花叶病毒(TMV)的作用。文章将TA3-13基因片段克隆到植物表达载体pBI121上,构建成转基因重组体pB-3-13,通过冻融法转化农杆菌EHA105,构建成转基因侵染菌株。采用叶盘法将pB-3-13转化三生烟草,经卡那霉素抗性筛选,获得48株T0代再生植株。通过PCR检测,鉴定出33株转基因单株,收获了20株种子作为T1代株系。PCR-Southern杂交结果显示,PCR阳性条带与TA3-13探针有特异性杂交,说明外源基因被转化到烟草的基因组中。选取两个T1代株系的烟草植株用于各项测定。GUS组织化学活性鉴定和RT-PCR检测结果显示,外源基因可以成功地表达。接种TMV病毒后,转基因烟草抗TMV的能力较转空载体烟草提高3～5倍。转基因烟草具有抗TMV侵入和抗病毒病害发展的作用,同时转基因烟草可以抗细菌软腐病菌的扩展。
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| 关 键 词： | TA3-13基因 转基因烟草 抗病性 冷胁迫蛋白 |
Study on the transgenic tobacco expressing truncated wheat cold shock protein gene TA3-13 and analysis of disease resistance |
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| Li N,DU XZ,Pan XM,Wang JS,Song CF.Study on the transgenic tobacco expressing truncated wheat cold shock protein gene TA3-13 and analysis of disease resistance[J].Hereditas,2011,33(5):520-526. |
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| Authors: | Li Na DU Xiu-Zhen Pan Xiao-Mei Wang Jin-Sheng Song Cong-Feng |
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| Institution: | LI Na,DU Xiu-Zhen,PAN Xiao-Mei,WANG Jin-Sheng,SONG Cong-Feng College of plant protection/Key Laboratory of Integrated Management of Crop Diseases and Pests,Ministry of Education,Nanjing Agri-cultural University,Nanjing 210095,China |
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| Abstract: | TA3-13 is a truncated gene coding for the fragment of wheat(Triticum aestivum L.) cold shock protein WCP1.It has been shown previously that the procaryotically expressed TA3-13 can induce resistance to Tobacco mosaic virus(TMV) when sprayed onto plant leaves.In this study,we constructed an expression vector pB-3-13 by cloning TA3-13 into the bionary vector pBI121 and transformed it into Agrobacterium tumefaciense strain EHA105 via freeze-thaw method.To-bacco(Nicotiana tobacum cv.Xanthi nc.) transformation w... |
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| Keywords: | TA3-13 gene transgenic tobacco disease resistance cold shock protein |
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