The Functional Quality of Soluble Recombinant Polypeptides Produced in Escherichia coli Is Defined by a Wide Conformational Spectrum |
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| Authors: | M nica Martí nez-Alonso, Nuria Gonz lez-Montalb n, Elena Garcí a-Fruit s, Antonio Villaverde |
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| Affiliation: | Mónica Martínez-Alonso, Nuria González-Montalbán, Elena García-Fruitós, and Antonio Villaverde |
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| Abstract: | We have observed that a soluble recombinant green fluorescent protein produced in Escherichia coli occurs in a wide conformational spectrum. This results in differently fluorescent protein fractions in which morphologically diverse soluble aggregates abound. Therefore, the functional quality of soluble versions of aggregation-prone recombinant proteins is defined statistically rather than by the prevalence of a canonical native structure. |
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