Induction of alkaline phosphatase activity in HeLa cells by sodium butyrate |
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Authors: | Walker Wharton Cathryn A. Hart Barry Goz |
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Affiliation: | (1) Department of Pharmacology, School of Medicine, University of North Carolina, 27514 Chapel Hill, North Carolina;(2) Present address: Group W, Cancer Research Center, University of North Carolina, 514 Swing Building 217H, 27514 Chapel Hill, North Carolina |
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Abstract: | Summary The induction of HeLa cell alkaline phosphatase activity by sodium butyrate could be inhibited by the coadministration of caffeine or theophylline. The inhibitions were dose dependent, and at any given concentration the potency was theophylline > caffeine. Although the induction by sodium butyrate was more sensitive to the inhibition by the xanthines than was that produced by 5-iodo-2′-deoxyuridine, the magnitudes of the increases in cyclic AMP concentrations after treatment with the xanthines were similar in the inhibition of both types of induction. The induction of alkaline phosphatase activity by sodium butyrate also produced a shift in the thermostability pattern of the enzyme, with a proportionately greater increase in the heat-labile, rather than heat-stable, from of the activity. Supported by National Cancer Institute Grant CA16460. |
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Keywords: | alkaline phosphatase HeLa cells sodium butyrate 5-iodo-2′ -deoxyuridine cyclic AMP |
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