Chlorophyll fluorescence imaging for disease-resistance screening of sugar beet |
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Authors: | Laury Chaerle Dik Hagenbeek Erik De Bruyne Dominique Van Der Straeten |
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Institution: | (1) Unit Plant Hormone Signalling and Bio-imaging, Ghent University, K.L. Ledeganckstraat 35, Gent, 9000, Belgium;(2) Phytopathology Department, SESVanderHave, Soldatenplein Z2, Industriepark 15, Tienen, 3300, Belgium |
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Abstract: | Both biotic and abiotic stresses cause considerable crop yield losses worldwide (Chrispeels, Sadava Plants, genes, and crop
biotechnology 2003; Oerke, Dehne Crop Prot 23:275–285 2004). To speed up screening assays in stress resistance breeding,
non-contact techniques such as chlorophyll fluorescence imaging can be advantageously used in the quantification of stress-inflicted
damage. In comparison with visual spectrum images, chlorophyll fluorescence imaging reveals cell death with higher contrast
and at earlier time-points. This technique has the potential to automatically quantify stress-inflicted damage during screening
applications. From a physiological viewpoint, screening stress-responses using attached plant leaves is the ideal approach.
However, leaf growth and circadian movements interfere with time-lapse monitoring of leaves, making it necessary to fix the
leaves to be studied. From this viewpoint, a method to visualise the evolution of chlorophyll fluorescence from excised leaf
pieces kept in closed petri dishes offers clear advantages. In this study, the plant–fungus interaction sugar beet–Cercospora beticola was assessed both in attached leaf and excised leaf strip assays. The attached leaf assay proved to be superior in revealing
early, pre-visual symptoms and to better discriminate between the lines with different susceptibility to Cercospora. |
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Keywords: | Cercospora beticola Sacc Chlorophyll fluorescence imaging Plant disease resistance quantification Plant– pathogen interaction Sugar beet Thermography |
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