Pyruvate and nitrogenase activity in cell-free extracts of the blue-green alga Anabaena cylindrica |
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Authors: | G A Codd P Rowell W D Stewart |
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Affiliation: | Department of Biological Sciences, University of Dundee, Dundee DD1 4HN, Scotland, U.K. |
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Abstract: | When extracts of are prepared in the absence of dithionite, they catalyze pyruvate-dependent acetylene reduction, a reaction not observable in assays containing dithionite. Ferredoxin and coenzyme-A, but not NADP and ferredoxin-NADP reductase, are required for maximal pyruvate-dependent activity. These acetylene-reducing extracts do not exhibit NADP-pyruvate dehydrogenase activity. However, pyruvate:ferredoxin oxidoreductase is present at levels of activity sufficient to support the rate of pyruvate-supported acetylene reduction. These data support earlier evidence that pyruvate:ferredoxin oxidoreductase transfers electrons from pyruvate to nitrogenase in . |
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