SrfI, a new type-II restriction endonuclease that recognizes the octanucleotide sequence, [sequence: see text] |
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| Authors: | T G Simcox S J Marsh E A Gross W Lernhardt S Davis M E Simcox |
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| Affiliation: | Stratagene Cloning Systems, Inc., La Jolla, CA 92037. |
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| Abstract: | A new restriction endonuclease, SrfI has been isolated from an unidentified species of Streptomyces. SrfI recognizes the 8-bp palindrome, 5'-GCCCGGGC and cleaves double-stranded DNA after the third C in the sequence, producing blunt ends. SrfI is a rare-cutting enzyme and should therefore be useful for megabase mapping. |
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